Rat Brain Tyrosine Hydroxylase

Regulatory properties of soluble and particulate rat brain tyrosine hydroxylase.

Rat brain tyrosine hydroxylase exists in two distinct physical forms, a soluble and a membrane-bound form, The two forms are brain-region specific, with the membranebound or particulate form predominating in the striatum, an area enriched in catecholamine nerve endings and associated with a specific membrane fraction. Whereas the soluble and the particulate enzymes exhibit an identical Km for t...

Dephosphorylation of purified brain tyrosine hydroxylase by rat striatal extracts.

Tyrosine hydroxylase (TH) was purified from bovine brain and enzymatically phosphorylated in vitro. Radioactively phosphorylated TH was dephosphorylated by rat tissue extracts. Of tissues examined, rat corpus striatal extracts were highest in specific activity in the TH dephosphorylating assay. Phosphorylated histone did not inhibit dephosphorylation of TH by rat striatal extracts. The thermal ...

Activation of Rat Caudate Tyrosine Hydroxylase Phosphatase

Tyrosine hydroxylase phosphatase activity in rat caudate nucleus was separated into three peaks by chromatography on DEAE-cellulose. [S2P]Tyrosine hydroxylase phosphorylated by cyclic AMP-dependent protein kinase was dephosphorylated only by the major peak eluting at 0.3 M NaCl, while tyrosine hydroxylase phosphorylated by Ca2+-calmodulin-dependent protein kinase was also dephosphorylated by tw...

Tyrosine hydroxylase purification from rat PC12 cells.

Tyrosine hydroxylase was purified in high yield from rat PC12 cells. This three-day procedure consisted of differential ammonium sulfate precipitation, anion-exchange chromatography, and heparin-Sepharose affinity chromatography. It yielded an average of 15 mg of purified protein from 100 flasks of PC12 cells, with greater than 40% recovery of tyrosine hydroxylase. Sodium dodecyl sulfate-polyac...

Phenylalanine Hydroxylase, Tyrosine Hydroxylase, and Tryptophan Hydroxylase